B16-F10 cells
Mouse B16-F10 cells were isolated from a spontaneous melanoma developing behind the ear of a C57BL/6 mouse.
Tumour growth in vivo
The cells were collected from a tissue culture flask and injected subcutaneously in the right flank of C57BL/6 mice. The resulting tumours were monitored by measuring two diameters with calipers, and extrapolating the volume to a sphere.
The mice bearing B16-F10 tumours can be treated by intra-peritoneal, intra-venous, intra-tumoral or subcutaneous injection of the compounds. Per os administration is also possible.